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Available files

We release all files concerning the analysis of the multi-stained complex sample of PBMC.

  • Input Data as an FCS file ("Cell_A" sample)
  • R scripts that were used to generate the data analyzed throughout the manuscript and link flowClust output with MeV visualisation.
  • Data generated with flowClust as
    • RData objects (runs on the range 1-30, 31-45, 46-55, 56-60).
    • FCS files for mixture with K=23, K=38, and K=60 clusters (for further visualisation with FlowJo).
  • AnalysisOfSet pseudo-code shows how to process a set of FCS files with flowClust and to export a MeV ready file.
  • Alternatively files could be browsed directly.
  • flowCL report contains the R code along with the results while querying some typical signatures. The code takes advantages of the flowCL package that is an interface to the Cell Ontology. The results show that some signature are clearly identified, but the wording in order to convert the MFI to levels (minus, plus, lo, hi) is important as well as the number of marker included in the query.
All scripts are provided under CeCILL Licence.